PDK1
| Cat Number: | AB-90230 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1. |
| Reactivity: | HU, Ms, |
| Applications: | Western Blot: 1:500 -1:1000 |
| Molecular Weight: | 68kDa |
| Purification: | Purified |
| Synonyms: | PDPK1; PDK1; PDPK2; PDPK2P; PRO0461; 3-phospho inositide-dependent protein kinase 1 |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using PDK1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico. .
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using PDK1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PDK1 antibody Blue: DAPI for nuclear staining.