Phospho-4E-BP1 (T37/46) Rabbit Monoclonal Antibody
| Cat Number: | MAB-94708 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | 236B4 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic phosphorylated peptide around T37 & T46 of human 4E-BP1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:500 – 1:2000 |
| Molecular Weight: | 15kDa-20kDa |
| Purification: | Affinity purification |
| Synonyms: | 4E-BP1;4EBP1;BP-1;PHAS-I;EIF4EBP1 |
| Background: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Phospho-4EBP1 (T37/46) antibody at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit
Exposure time: 30s.
Western blot analysis of extracts of C6 cells, using Phospho-4EBP1 (T37/46) antibody at 1:1000 dilution. C6 cells were treated by by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit
Exposure time: 180s.
Immunohistochemistry of paraffinembedded rat kidney using Phospho-4EBP1 (T37/46) antibody at dilution of 1:100 (40x lens). Perform high
pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.