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Phospho-ATF4-(S245)

Cat Number: ABP-0309
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A phospho specific peptide corresponding to residues surrounding S245 of human ATF4
Reactivity: Hu
Applications:

Western blot: 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1: 50-1:100

Molecular: 45kDa
Purification: Affinity purification
Background:

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP-0309-1.jpg

Western blot analysis of extracts from Hela cells using Phospho-ATF4-S245 antibody.

ABP-0309-2.jpg

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-ATF4-S245 antibody