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Phospho-ATM-(S1981)

Cat Number: ABP-0008
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic phosphorylated peptide around S1981 of human ATM
Reactivity: Hu
Applications:

Western Blot: 1:500 – 1:2000 Immunofluorescence: 1:50 -1:200

Molecular: 350kDa
Purification: Affinity purification
Synonyms: ATM; AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO 1; serine-protein kinase ATM
Background:

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP-0008-1.jpg

Western blot analysis of extracts of 293T cells, using Phospho-ATM-S1981 antibody at 1:2000 dilution. 293T cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus Exposure time: 10s

ABP-0008-2.jpg

Immunofluorescence analysis of MCF-7 cells using Phospho-ATM-S1981 antibody . Blue: DAPI for nuclear staining.

ABP-0008-3.jpg

Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody Blue: DAPI for nuclear staining.

ABP-0008-4.jpg

Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody Blue: DAPI for nuclear staining.