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Phospho-Axl (Y691) Rabbit Polyclonal Antibody

Cat Number: ABP10101
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human AXL around the phosphorylation site of Tyr691. AA range:657-706
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1/500 – 1/2000
Immunohistochemistry: 1/100 – 1/300
Elisa: 1/20000
Molecular Weight: 130kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: AXL; UFO; Tyrosine-protein kinase receptor UFO; AXL oncogene
Background:

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two
immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin
K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types.
Alternative splicing results in multiple transcript variants of this gene.
Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
ABP10101-1

Western Blot analysis of HuvEc cells using Phospho-Axl (Y691) Polyclonal Antibody diluted at 1:500

ABP10101-2

Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 15′, using AXL (Phospho-Tyr691)
Antibody. The lane on the right is blocked with the phospho peptide.

ABP10101-3

Immunohistochemistry analysis of paraffin-embedded human brain, using AXL (Phospho-Tyr691) Antibody. The picture on the right is blocked with the phospho peptide.