Phospho-Beclin-1 (S93) Rabbit Monoclonal Antibody
| Cat Number: | MAB-94690 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | D9A5G |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic phosphorylated peptide around S93 of human Beclin 1 |
| Reactivity: | Hu |
| Applications: | WB 1:500 – 1:2000 |
| Molecular Weight: | 60KDa |
| Purification: | Affinity purification |
| Synonyms: | ATG6; VPS30; beclin1 |
| Background: | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the |
| Form: | liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of HeLa cells, using Phospho-Beclin 1-Ser93 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.