Phospho-Beclin-1 (S93) Rabbit Monoclonal Antibody

Cat Number: MAB-94690
Conjugate: Unconjugated
Size: 100 ug
Clone: D9A5G
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic phosphorylated peptide around S93 of human Beclin 1
Reactivity: Hu

WB 1:500 – 1:2000

Molecular Weight: 60KDa
Purification: Affinity purification
Synonyms: ATG6; VPS30; beclin1

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the
phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Form: liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of HeLa cells, using Phospho-Beclin 1-Ser93 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.