Phospho-Crk-L (Y207)
| Cat Number: | ABP10285 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic phosphorylated peptide around Y207 of human CRKL. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 Immunohistochemistry: 1:50 – 1:100WB, IHC |
| Molecular Weight: | 38kDa |
| Purification: | Affinity Purification |
| Background: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of K-562 cells, using Phospho-CRKLY207 antibody at 1:1000 dilution or CRKL antibody. K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 5s.
Immunohistochemistry of paraffin embedded rat brain using Phospho- CRKL-Y207 antibody at dilution of 1:100 (40x lens).