Phospho-DDX3 (T322)
| Cat Number: | ABP10322 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human DDX3 around the phosphorylation site of T322. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:10000 |
| Molecular Weight: | 73kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen. |
| Background: | Phospho-DDX3 (T322) Polyclonal Antibody detects endogenous levels of DDX3 protein only when phosphorylated at T322. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at-20°C, and avoid repeat freeze-thaw cycles |
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.
Immunofluorescent analysis of Phospho-DDX3 pThr322 in serum-treated HUVEC cells using a Phospho-DDX3 pThr322 polyclonal antibody.