Phospho-eIF2α (S51) Rabbit Monoclonal Antibody
| Cat Number: | MAB-94689 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | D9G8 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic phosphorylated peptide around S51 of human eIF2a |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:500 – 1:2000 |
| Molecular Weight: | 38 kDa |
| Purification: | Affinity purification |
| Synonyms: | EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1 |
| Background: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3 |
| Storage: | Store at –20°C. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 Rabbit MAb at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.