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Phospho-IGF-1R (Y1161)

Cat Number: ABP-0367
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human IGF-IR around the phosphorylation site of Y1161. Immunogen Region: 1100-1180 aa
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:1000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000

Molecular: 95 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Background:

Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.Specificity: Phospho-IGF-IR (Y1161) Polyclonal Antibody detects endogenous levels of IGF-IR protein only when phosphorylated at Y1161.

Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
ABP-0367-1.jpg

Western Blot (WB) analysis of specific cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody.

ABP-0367-2.jpg

mmunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for anbitody retrieval. Negetive contrl (right) obtaned from antibody was pre-abso

ABP-0367-3.jpg

Immunofluorescence staining – Phospho-IGF1R-Y1161 antibody