Phospho KAT-5 (Ser 86) Rabbit Polyclonal Antibody
| Cat Number: | ABP11224 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TIP60 around the phosphorylation site of Ser86. AA range:52-101 |
| Reactivity: | Human;Mouse |
| Applications: | Western Blot: 1:500-2000 |
| Molecular Weight: | 65kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | KAT5; HTATIP; TIP60; Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein |
| Background: | The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody
Western blot analysis of lysates from Jurkat cells, using TIP60 (Phospho-Ser86) Antibody.
The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer.
1, Antibody was diluted at 1:200(4° overnight).
2, Tris-EDTA,pH9.0 was used for antigen retrieval.
3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIP60 (Phospho-Ser86) Antibody