Phospho KAT-5 (Ser 86) Rabbit Polyclonal Antibody

Cat Number: ABP11224
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human TIP60 around the phosphorylation site of Ser86. AA range:52-101
Reactivity: Human;Mouse

Western Blot: 1:500-2000
Immunohistochemistry (paraffin-embedded tissues): 1:50-300
ELISA 2000-20000

Molecular Weight: 65kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: KAT5; HTATIP; TIP60; Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.

Western Blot analysis of Jurkat cells using Phospho-TIP60 (S86) Polyclonal Antibody


Western blot analysis of lysates from Jurkat cells, using TIP60 (Phospho-Ser86) Antibody.
The lane on the right is blocked with the phospho peptide.


Immunohistochemical analysis of paraffin-embedded human Colon cancer.
1, Antibody was diluted at 1:200(4° overnight).
2, Tris-EDTA,pH9.0 was used for antigen retrieval.
3,Secondary antibody was diluted at 1:200(room temperature, 45min).


Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIP60 (Phospho-Ser86) Antibody