Phospho-MAPKAPK-2 (T334)
Cat Number: | ABP-0396 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | Poly |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of T334 |
Reactivity: | Hu, Ms, Rt |
Applications: | Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:20000 |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen. |
Background: | Phospho-MAPKAPK-2 (T334) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T334. |
Form: | liquid |
Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Storage: | Store at-20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is blocked with the phospho peptide.