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Phospho-MAPKAPK-2 (T334)

Cat Number: ABP-0396
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of T334
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Background:

Phospho-MAPKAPK-2 (T334) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T334.

Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at-20°C, and avoid repeat freeze-thaw cycles.
ABP-0396-1.jpg

Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide

ABP-0396-2.jpg

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

ABP-0396-3.jpg

Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is blocked with the phospho peptide.