Phospho-MAPKAPK-2 (T334)
| Cat Number: | ABP-0396 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human MAPKAPK-2 around the phosphorylation site of T334 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:20000 |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen. |
| Background: | Phospho-MAPKAPK-2 (T334) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T334. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at-20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of lysates from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) Antibody. The lane on the right is blocked with the phospho peptide
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Thr334) Antibody. The picture on the right is blocked with the phospho peptide.