Phospho-P70 S6K1 (T389) Rabbit Monoclonal Antibody
| Cat Number: | MAB-94648 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | 108D2 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic phosphorylated peptide around T389 of human P70 S6K |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:500 – 1:2000 |
| Molecular Weight: | 70 kDa |
| Purification: | Affinity purification |
| Synonyms: | PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA |
| Background: | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of NIH/3T3, using Phospho-p70 S6K1 (T389) antibody at 1:500 dilution. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 180s.
Immunofluorescence analysis of C6 cells using Phospho-p70 S6K1 (T389) antibody at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.
Immunofluorescence analysis of HeLa cells using Phospho-p70 S6K1 (T389) antibody at dilution of 1:100.
HeLa cells were treated by Serum-starvation overnight at 37℃.