Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:10000

PI 3-kinase p85alpha is a protein encoded by the PIK3R1 gene which is approximately 83,5 kDa. PI 3-kinase p85alpha is localised to the cytoplasm, cytosol and nucleus. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2, regulation of lipid metabolism and insulin signalling-generic cascades. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter in mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85alpha isoform 2 and isoform 4 are present in skeletal muscle. Mutations in the PIK3R1 gene may result in Short syndrome and Agammaglobulinemia. ABP10906 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous PI 3-kinase p85alpha protein which only binds about Y607 when Y607 is phosphorylated.Phospho-PI 3-kinase p85alpha (Y607) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85alpha protein only when phosphorylated at Y607.Tissue Specificity: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).