Phospho-PKC (pan) (βII Ser660)
| Cat Number: | ABP-0495 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) βII |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:1000 |
| Molecular Weight: | 95kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (βII Ser660) antibody at 1:1000 dilution. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECLWest Pico Plus. Exposure time: 90s.