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Phospho-DNA-PKcs-S2056 Rabbit Polyclonal Antibody

Cat Number: ABP10327
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic phosphorylated peptide around S2056 of human DNA-PKcs.
Reactivity: Human
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200

Molecular Weight: 469kDa
Purification: Affinity purification
Synonyms: HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs
Background:

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

Form: liquid
Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP-0621-1

Western blot analysis of extracts of HeLa cells, using Phospho-DNA-PKcs-S2056 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABP-0621-2.jpg

Immunofluorescence analysis of U2OS cells using Phospho-DNA-PKcs-S2056 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.

References:

References for Phospho-DNA-PKcs-S2056 Rabbit pAb
Product: Phospho-DNA-PKcs-S2056 Rabbit pAb
Journal: mBio
Application: WB
IF: 0
Species: Homo sapiens
PMID: 36719192
Title: Adeno-Associated Virus Monoinfection Induces a DNA Damage Response and DNA Repair That Contributes to Viral DNA Replication