Phospho-SMAD1 SER 463/465+SMAD5 Ser463/465+SMAD9 Ser465/467)
| Cat Number: | ABP-0850 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S463 of human Smad1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 60 kDa |
| Purification: | Affinity purification |
| Synonyms: | Smad1/Smad5/Smad9 |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of HeLa cells, using PhosphoSmad1(Ser463/465)/Smad5(Ser463/465)/Smad 9(Ser465/467) Polyclonal antibody at 1:1000 dilution.HeLa cells were treated by ATP (5 mM) at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 60s.