Phospho-Stat1 (Tyr701) Rabbit Polyclonal Antibody
| Cat Number: | ABP-0135 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717. |
| Reactivity: | Human;Mouse;Rat;Monkey |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 87 kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84 |
| Background: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X ,3) COS7 cells, （Green）primary antibody was
diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour.
（Red） Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4° over
night,secondary antibody was diluted at 1:10000, 37° 1hour.
Immunofluorescence analysis of Hela cell. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at
1:200(4° overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4° overnight).
2, Goat Anti Rabbit Alexa Fluor 594
Immunofluorescence analysis of rat-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at
1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of rat-spleen tissue.
1,Stat1 (phospho Tyr701) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min.
Picture A:Target.
Picture B: DAPI.
Picture C: merge of A+B