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Phospho-Stat1 (Y701)

Cat Number: ABP-0135
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human Stat1 around the phosphorylation site of Y701. Immunogen Region 640-720 aa
Reactivity: Hu, Ms, Rt, Monkey
Applications:

Western Blot: 1:1000 Immunohistochemistry (paraffin embedded tissues) 1:200 Immunoprecipitation 1: 300 ELISA 1:10,000

Molecular: 87 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity- chromatography using epitope-specific immunogen.
Synonyms: Phospho-Stat1 (Y701) Signal transducer and activator of transcription 1-alpha/beta antibody Transcription factor ISGF-3 components p91/p84 antibody
Background:

Stat1 is a protein encoded by the STAT1 gene which is approximately 87,3 kDa. Stat1 is localised to the cytoplasm and nucleus. It is involved in common cytokine receptor gamma-chain family signalling pathways, Th17 cell differentiation, toll-like receptor signalling pathways and the prolactin signalling pathway. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the expression of a variety of genes and is thought to be important for cell viability in response to different cell stimuli and pathogens. Stat1 is expressed in the nervous system, liver, skin, blood and lymph node. Mutations in the STAT1 gene may result in immunodeficiency This antibody was affinity- purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Stat1 protein which only binds about Y701 when Y701 is phosphorylated. Phospho-Stat1 (Y701) Polyclonal Antibody detects endogenous levels of Stat1 protein only when phosphorylated at Y701.

Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
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Western blot (WB) analysis of Stat1 (p Tyr701) on mouse brain

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IHC analysis of mouse liver tissues 1:200 (4°C overnight ) antigen Retrieval – Sodium Citrate pH6 (>98°C , 20 min) Secondary 1:200 (RT 30 min) – negative control secondary only

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Immunohistochemical analysis of mouse spleen tissue. Anti-Phospho-Stat1 (Y701) at 1:200 (4°C,overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min). Negative control – Secondary only

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Immunohistochemical analysis of rat lung tissue. Anti-Phospho-Stat1 (Y701) at 1:200 (4°C,overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min). Negative control – Secondary only