Phospho-Stat3 (Y705)
| Cat Number: | ABP-4001 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human Stat3 around the phosphorylation site of Tyr705. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:200 Immunofluorescence: 1:100 Immunoprecipitation: 1-2,5 ug/mg lysate ELISA: 1:5000 |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Background: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.Phospho-Stat3 (Tyr705) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705 |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at –20°C. Do not aliquot the antibody. |
Western Blot analysis of our customer’s samples using primary antibody diluted at 1:500, The picture was kindly provided by our customer.
Western Blot analysis of various cells using Phospho-Stat3 (Tyr705) Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of mouse-spleen tissue. A) Stat3 (phospho Tyr705) Polyclonal Antibody(red) was diluted at 1:100(4°C,overnight). B) Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).C) Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IHC(P) on Human-lung-cancer tissue. Stat3 (phospho Tyr705) 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody at 1:200(RT 30min). Negative control was used by secondary antibody only.
IHC(P) Human-Appendix tissue. Stat3 (phospho Tyr705) 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). Secondary antibody was diluted at 1:200(RT 30min). Negative control was used by secondary antibody only.
IHC(P) Rat-lung tissue. Stat3 (phospho Tyr705) 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). Secondary antibody was diluted at 1:200 (RT, 30min). Negative control was used by secondary antibody only.