Top

Phospho-TrkA (Tyr496) Rabbit Polyclonal Antibody

Cat Number: ABP-11213
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized pospho peptide derived from human Trk A (Phospho-Tyr496)
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:100 – 1:500
ELISA: 1:5000 – 1:20000
Molecular Weight: 180 kDa
Purification: Affinity Purified
Synonyms: NTRK1; MTC; TRK; TRKA
Background:

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes.
Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Form: Liquid
Buffer: PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
ABP-11213-3

Western blot analysis of various lysates. Primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

ABP-11213-1

Immunofluorescence analysis of NIH/3T3 cells, using Phospho-TrkA (Tyr496) Antibody. The picture on the right is blocked with the phospho peptide.

ABP-11213-2

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for
Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Phospho-TrkA (Tyr496) Antibody