PLK1 Rabbit Polyclonal Antibody
| Cat Number: | AB-83900 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from Plk1 . at AA range: 80-160 |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western Blot: 1/500 – 1/2000. |
| Molecular Weight: | 68kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13 |
| Background: | The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen
retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.