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PTCH1 Rabbit Polyclonal Antibody

Cat Number: AB-81569
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human Patched. AA range:1-50
Reactivity: Human, Mouse
Applications:

Western Blot: 1/500 – 1/2000
Immunohistochemistry: 1/100 – 1/300
Molecular Weight: 160kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: PTCH1; PTCH; Protein patched homolog 1; PTC; PTC1
Background:

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in
tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.
Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20℃. Avoid repeated freeze-thaw cycles.
AB-81569-1

Western blot analysis of lysates from mouse muscle cells, using Patched Antibody. The lane on the right is blocked with the synthesized peptide.

AB-81569-2

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).