Recombinant Anti-beta Actin Rabbit Monoclonal Antibody
| Cat Number: | MABS15003 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide corresponding to Mouse beta-Actin |
| Reactivity: | Species, Human, Mouse, Rat, Pg, Ch, Bv, Sh, Gt, Mk, Rb, Ta, Frog, Human, Mouse, Rat |
| Applications: | Applications Dilution WB 1: 3000-1: 5000 IHC/IF 1: 500-1: 1000 ICC/IF 1: 300-1: 1000 |
| Molecular: | 42 kDa / 42 kDa |
| Purification: | Affinity purification |
| Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
| Background: | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. |
| Form: | Liquid |
| Buffer: | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of beta Actin . Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.