|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100B|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:500- 1: 2000
|Synonyms:||S100B; NEF; S100; S100-B; S100beta; protein S100-B|
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer’s disease, Down’s syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20°C. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of various cell lines, using S100B antibody at a dilution of 1:1000.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunofluorescence analysis of U-251MG cells using S100B antibody – dilution 1:100 – Blue: DAPI for nuclear staining
Immunofluorescence analysis of of mouse brain using S100B antibody – dilution: 1:100
Blue: DAPI for nuclear staining