S1PR1
| Cat Number: | AB-83739 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant funsion protein containing a sequence of human S1PR1 |
| Reactivity: | Hu, Ms |
| Applications: | Western Blot: 1:500- 1:1000 |
| Molecular Weight: | 43kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CD363;CHEDG1;D1S3362;ECGF1;EDG-1;EDG1;S1P1 |
| Background: | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot using S1PR1 on extracts of
various cell lines at 1:1000 dilution.
Secondary antibody:
HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk inTBST.
Detection: ECL West Pico Kit.
Exposure time: 90s.
Immunofluorescence: 1:200
Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 Antibody. The picture on the right is blocked with the synthesized peptide