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SAM68

Cat Number: AB-84326
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the Cterminus of human SAM68.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:100 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 68kDa
Purification: Aff. Pur.
Synonyms: KHDRBS1;Sam68;p62;p68
Background:

This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3′-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using SAM68 antibody ) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL west Pico Plus.
Exposure time: 10s.

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Immunohistochemistry of paraffin embedded
rat brain using SAM68
antibody at dilution of 1:100 (40x lens).

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Confocal immunofluorescence analysis of
HeLa cells using SAM68Polyclonal
Antibody at dilution of 1:400. Blue:
DAPI for nuclear staining.

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Review for SAM68 Rabbit pAb
Experimen Type: Immunofluorescence(IF)
Sample: Zebrafish retina
Description: Immunofluorescence analysis of Zebrafish retina, using SAM68 Polyclonal Antibody.

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Review for SAM68 Rabbit pAb
Experiment Type: Immunofluorescence(IF)
Sample: Zebrafish retina
Description: Immunofluorescence analysis of Zebrafish retina, using PCBP2 Polyclonal Antibody.