SDHB
| Cat Number: | MAB-94613 |
|---|---|
| Size: | 100 ug |
| Clone: | 21A11AE7 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 32kDa |
| Purification: | Aff. Pur |
| Synonyms: | CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHIP |
| Background: | Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using SDHB antibody
at 1:1000 dilution.
Secondary antibody: HRP
Goat Anti-Rabbit IgG (H+L) at
1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.Exposure time: 1s.
Immunohistochemistry
of paraffin embedded
human colon using SDHB
antibody at dilution of 1:100 (40x
lens).
Immunohistochemistry of paraffin embedded
mouse spleen using SDHB
antibody at dilution of 1:100 (40x
lens).
Immunofluorescence analysis of U2OS
cells using SDHB Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear
staining.
Immunofluorescence analysis of L929 cells using SDHB Rabbit mAb at dilution of 1:100.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using SDHB Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.