SLUG
| Cat Number: | AB-84208 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 89-268 of human SLUG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot : 1:500-1:2,000 |
| Molecular Weight: | 29kDa |
| Purification: | Aff. Pur. |
| Synonyms: | SNAI2; SLUG; SLUGH; SLUGH1; SNAIL2; WS2D |
| Background: | This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. |
| Form: | Liquid |
| Buffer: | PBS with 0,02% sodium azide, 50% glycerol pH7.3 |
| Storage: | Storage for short term at 4°C recommended, for longer term at -20°C, minimize freeze/thaw cycles |
Western blot analysis of extracts of various cell lines, using SLUG antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit
Exposure time: 60s..
Immunohistochemistry of paraffin-embedded rat brain using SLUG Antibody at dilution of 1:100
Application: Western blot (WB)
Sample mouse cells
Loading Amount 15 ug
Dilution 1:1000
Incubation time over night
Temperature : 4 ℃
Exposure: 30s
Observed Band: 25 kDa
Application: Western blot Sample MDA-MB-231
Loading Amount 15 ug
Dilution 1:2000
Incubation time over night
Temperature 4 °C
Exposure 30s
Observed Band 29 kDa
Immunofluorescence analysis of A549 cells using SLUG antibody. Blue: DAPI for nuclear staining.
References
Product:SLUG Polyclonal Antibody Journal:Oncology Application:WB IF:2.262
Species:human PMID:29566977
Title:
CO suppresses prostate cancer cell growth by directly targeting LKB1/AMPK/mTOR pathway in vitro and in vivo
Product:SLUG Polyclonal Antibody -Journal:Theranostics Application:WB, IF IF:8.688
Species:Human PMID 30026878
Title:
AP-2β inhibits hepatocellular carcinoma invasion and metastasis through Slug and Snail to suppress epithelial-mesenchymal transition
Product:SLUG Polyclonal Antibody Journal:Toxicology and applied pharmacology
Application:WB, IF, Co-IP, IF:
Species:Human – PMID:31381904
Title:
The involvement of lipid raft pathway in suppression of TGFβ-mediated metastasis by tolfenamic acid in hepatocellular carcinoma cells.
Product:SLUG Polyclonal Antibody -Journal:Cellular signalling
Application:WB, IF, IP IF:3.48 Species:Human
PMID:30953699 –
Title:
Effects of MCRS1 on proliferation, migration, invasion, and epithelial mesenchymal transition of gastric cancer cells by interacting with Pkmyt1 protein kinase.