Smad2/3
| Cat Number: | AB-90212 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human SMAD3. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 56-60kDa |
| Purification: | Aff. Pur. |
| Synonyms: | HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3 |
| Background: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic’ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factorbeta and is thought to play a role in the regulation of carcinogenesis. |
| Form: | Liquid |
| Buffer: | PBS with 0,02% sodium azide, 50% glycerol pH7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
HeLa cells, using SMAD2/SMAD3
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s.
Immunohistochemistry of paraffinembedded
human kidney using
SMAD2/SMAD3 antibody at
dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS
cells using SMAD2/SMAD3 antibody
at dilution of 1:100. Blue:
DAPI for nuclear staining.