Smoothened Rabbit Polyclonal Antibody
| Cat Number: | AB-84784 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-233 of human SMO. |
| Reactivity: | Human, Mouse |
| Applications: | Western Blot: 1:100 – 1:500 |
| Molecular Weight: | 86kDa |
| Purification: | Affinity purification |
| Synonyms: | Gx; CRJS; PHLS; SMOH; FZD11; SMO |
| Background: | The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. |
| Form: | Liquid |
| Buffer: | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Western blot analysis of extracts of various cell lines, using SMO antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 120s.
Western blot analysis of extracts of Mouse brain, using SMO antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico plus.
Exposure time: 180s.