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STAT 3 Rabbit Polyclonal Antibody

Cat Number: AB-83813
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human STAT3. AA range:672-721
Reactivity: Hu, Ms,Rt
Applications:

Western Blot: 1/500 1/2000
Immunofluorescence: 1:50-200
Immunohistochemistry: 1/100 – 1/300
ELISA:1/5000

Molecular Weight: 88kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Background:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular
processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20℃. Avoid repeated freeze-thaw cycles.
AB-83813-1

Western blot analysis of various lysates, using STAT3 Rabbit pAb at 1:2500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

AB-83813-2

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb at dilution of 1:200 (40x lens).
Blue: DAPI for nuclear staining.

AB-83813-3

Immunohistochemistry analysis of paraffin-embedded mouse brain using STAT3 Rabbit pAb at dilution of
1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC
staining protocol.