SUMO 2/3 Rabbit Polyclonal Antibody
| Cat Number: | AB-84757 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-95 of human SUMO2/3. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:100 – 1:500 |
| Molecular Weight: | 17kDa |
| Purification: | Affinity purification |
| Synonyms: | HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2 |
| Background: | This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using SUMO2/3 Rabbit pAb at 1:500
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded rat ovary using SUMO2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC
staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal using SUMO2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-2 OS cells using SUMO2/3 Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
References:
Product: SUMO2/3 Rabbit pAb
Journal:eLife
Application:WB
IF:8.71
Species:Mus musculus
PMID:35942699
Title:Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy – PMC