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SUMO1 Rabbit Polyclonal Antibody

Cat Number: AB-84756
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-101 of human Sumo 1.
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry (paraffin-embedded tissues): 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Molecular Weight: 17kDa/80kDa
Purification: Affinity purification
Synonyms: DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Background:

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to
target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of
cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus
have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles..
AB-84756-1

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

AB-84756-2

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-84756-3

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1min.

AB-84756-4

Western blot analysis of extracts from wild type(WT) and SUMO1 knockout (KO) 293T cells, using
SUMO1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-84756-5

Immunohistochemistry analysis of paraffin-embedded human breast cancer using SUMO1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

AB-84756-6

Confocal imaging of NIH/3T3 cells using [KO Validated] SUMO1 Rabbit pAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue).
Objective: 100x.

References:

References for [KO Validated] SUMO1 Rabbit pAb
Journal:PLoS Pathog
Application:WB
IF:6.82
Species:Homo sapiens
PMID:34871326
Title:RSK1 SUMOylation is required for KSHV lytic replication References for [KO Validated] SUMO1 Rabbit pAb

Product:[KO Validated] SUMO1 Rabbit pAb
Journal:Technol Cancer Res Treat
Application:WB
IF:3.399
Species:Homo sapiens
PMID:34918563
Title:MicroRNA-133b Inhibits nTumor Cell Proliferation, Migration and Invasion by Targeting SUMO1 in Endometrial Carcinoma

References for [KO Validated] SUMO1 Rabbit pAb
Journal:PLoS pathogens
Application:WB
IF:7.46
Species:Homo sapiens
PMID:35482828
Title:The SUMO E3 ligase activity of ORF45 determines KSHV lytic replication

References for [KO Validated] SUMO1 Rabbit pAb
Journal:Molecular plant
Application:IP
IF:21.94
Species:N.benthamiana
PMID:36597359
Title:SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants