Synaptophysin
| Cat Number: | AB-82642 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Synaptophysin. |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 38kDa |
| Purification: | Aff. Pur. |
| Synonyms: | SYP; MRX96; MRXSYP; |
| Background: | This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Synaptophysin antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 20s.
Immunohistochemistry of paraffin embedded rat brain using Synaptophysin
antibody at dilution of 1:100
(40x lens).
Immunohistochemistry of paraffin embedded mouse spinal cord using Synaptophysin antibody at dilution of 1:100 (40x lens).