TAF15
| Cat Number: | AB-84381 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280of human TAF15 |
| Reactivity: | Hu |
| Applications: | WB: 1:500 – 1:2000 |
| Molecular Weight: | 80kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TAF15;Npl3;RBP56;TAF2N;TAFII68 |
| Background: | This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Form: | Liquid |
| Buffer: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using TAF15 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug TAF15 antibody.
Western blot was performed from the immunoprecipitate using TAF15 antibody at a dilition
of 1:1000.
Immunohistochemistry of paraffinembedded
human liver cancer using TAF15
Rabbit pAb at dilution of 1:150
(40x lens).