TDP43 Rabbit Polyclonal Antibody
Cat Number: | AB-83805 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human TDP-43/TARDB. |
Reactivity: | Human, Mouse, Rat |
Applications: | Western Blot: 1:500 – 1:2000 |
Molecular Weight: | 35kDa/45kDa |
Purification: | Affinity Purified |
Synonyms: | ALS10; TDP-43; TDP-43/TARDBP |
Background: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3..3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
![AB-83805-1](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-1-1.jpg)
Western blot analysis of various lysates using TDP-43/TARDBP Rabbit pab at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
![AB-83805-2](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-2-1.jpg)
Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit pAb
and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR.
Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
![AB-83805-3](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-3-1.jpg)
Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit pAb dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse pAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue).
Objective: 100x.
![AB-83805-4](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-4-2.jpg)
Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit pAb
at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![AB-83805-5](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-5.jpg)
Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded human esophageal using TDP-43/TARDBP
Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2
before commencing with IHC staining protocol.
![AB-83805-6](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-6.jpg)
Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![AB-83805-7](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83805-7-1024x1024.jpg)
Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded rat spleen using TDP-43/TARDBP Rabbit pAb
at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
before commencing with IHC staining protocol.