TFEB
| Cat Number: | AB-84280 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human TFEB. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular: | 70kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TFEB;ALPHATFEB;BHLHE35;TCFEB |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using TFEB
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using TFEB Rabbit pAb at
dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin embedded human colon using TFEB Rabbit pAb at dilution of 1:100 (40x lens).
Review for TFEB Rabbit pAb
References
TFEB Rabbit pAb
Product:TFEB Rabbit pAb
Journal:Cellular Signalling
Application:IF
IF:3.937
Species:Homo sapiens
PMID:28366813
Title:MITA modulated autophagy flux promotes cell death in breast cancer cells