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TFEB

Cat Number: AB-84280
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human TFEB.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 70kDa
Purification: Aff. Pur.
Synonyms: TFEB;ALPHATFEB;BHLHE35;TCFEB
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84280-1.jpg

Western blot analysis of extracts of
various cell lines, using TFEB
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus
Exposure time: 30s.

AB-84280-2.jpg

Immunofluorescence analysis of NIH-3T3 cells using TFEB Rabbit pAb at
dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.

AB-84280-3.jpg

Immunohistochemistry of paraffin embedded human colon using TFEB Rabbit pAb at dilution of 1:100 (40x lens).

AB-84280-4.jpg

Review for TFEB Rabbit pAb

References

TFEB Rabbit pAb
Product:TFEB Rabbit pAb
Journal:Cellular Signalling
Application:IF
IF:3.937
Species:Homo sapiens
PMID:28366813
Title:MITA modulated autophagy flux promotes cell death in breast cancer cells