TGF beta1
| Cat Number: | AB-84169 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 481-683 of human TGFBI |
| Reactivity: | Hu,Ms |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 75kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TGFBI; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CS D2; CSD3; EBMD; LCD1; transforming growth fact or beta induced. |
| Background: | This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using TGFBI
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin embedded
human liver cancer using
TGFBI antibody at dilution of
1:100 (40x lens).
Immunohistochemistry of paraffin embedded
human colon carcinoma
using TGFBI antibody at
dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin embedded
human lung cancer using
TGFBI antibody at dilution of
1:100 (40x lens).