TIMP-2 Rabbit Polyclonal Antibody
| Cat Number: | AB-90221 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TIMP2. AA range:21-70 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 25kDa |
| Purification: | Aff. Pur. |
| Synonyms: | TIMP2; Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2 |
| Background: | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of various cells using TIMP-2 Polyclonal Antibody diluted at 1:1000.
Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight).2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min)
Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval.3,Secondary antibody was diluted at 1:200(room temperature, 30min).