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TNF alpha Polyclonal Antibody

Cat Number: ABE3623
Conjugate: Unconjugated
Size: 100ug
Clone: Polyclonal
Concentration: 1 mg/mL
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from the Internal region of human TNF-α
Reactivity: Human;Mouse;Rat
Applications: Western Blot: 1:500-1:2000; Immunohistochemistry: 1:100-1:300;Immunofluorescence: 1:200-1:1000
Molecular: 16 kDa
Purification: Affinity purification
Synonyms: TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha
Background:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Buffer: Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Western Blot analysis of Jurkat cells with TNF alpha Polyclonal Antibody

Western Blot analysis of Jurkat cells with TNF alpha Polyclonal Antibody

Immunofluorescence analysis of Human stomach tissue using TNF alpha Polyclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Human stomach tissue using TNF alpha Polyclonal Antibody at dilution of 1:200.