Topoisomerase I Rabbit Polyclonal Antibody
| Cat Number: | ABN00094 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Clone: | Polyclonal |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of human Topoisomerase I |
| Reactivity: | Human,Mouse |
| Applications: | WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,FC 1:50-1:100 |
| Molecular: | Calculated MW: 91 kDa; Observed MW: 91 kDa |
| Purification: | Affinity Chromatography |
| Synonyms: | TOP1; DNA topoisomerase 1; DNA topoisomerase I |
| Background: | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5′-OH DNA strand. |
| Form: | Liquid |
| Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles. |