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TRKA

Cat Number: AB-83821
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TrkA
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry:1:50 – 1:200
Molecular Weight: 120 kDa
Purification: Aff. Pur.
Synonyms: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
Background:

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family.
This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes.
Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
AB-83821-1

Western blot analysis of extracts of various cell lines, using TrkA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 10s

AB-83821-2

Immunohistochemistry of paraffinembedded mouse stomach using TrkA Antibody at dilution of 1:100 (40xlens).
Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

AB-83821-3

Immunohistochemistry of paraffinembedded rat cerebellum using TrkA Antibody at dilution of 1:100 (40xlens).
Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol