TWIST1 Rabbit Monoclonal Antibody
| Cat Number: | MABN36693 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | Monoclonal |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG,Kappa |
| Immunogen: | Endogenous |
| Reactivity: | Human,Mouse, Rat |
| Applications: | WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000 |
| Molecular: | 26 kDa |
| Purification: | Protein A |
| Synonyms: | BHLHA38 TWIST |
| Background: | Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
| Form: | liquid |
| Buffer: | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% Protective protein |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution .
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was
blotted with anti-TWIST1 antibody. The HRP Goat anti-Rabbit IgG(H + L) antibody was
used to detect the antibody.
Immunofluorescence analysis of HeLa .
Picture A: TWIST1 Rabbit mAb (red).
Picture B: DAPI (blue).
Picture C: Merge of A+B