VEGFB Rabbit Polyclonal Antibody
Cat Number: | AB-82949 |
---|---|
Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Synthetic peptide corresponding to a sequence within amino acids 40-100 of mouse VEGFB |
Reactivity: | Hu, Ms, Rt |
Applications: | WB 1:1000 – 1:2000 |
Molecular Weight: | 32kDa |
Purification: | Aff. Pur. |
Synonyms: | VEGFB;VEGFL;VRF;VEGF-B |
Background: | This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclearencoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. |
Form: | Liquid |
Buffer: | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
![AB-82949-1.jpg](https://immunologicalsciences.com/wp-content/uploads/2023/02/AB-82949-1.jpg)
Western blot analysis of extracts of various cell lines, using VEGFB antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
![AB-82949-2.jpg](https://immunologicalsciences.com/wp-content/uploads/2023/02/AB-82949-2.jpg)
Immunohistochemistry of paraffinembedded rat kidney using VEGFB Rabbit Ab at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
![AB-82949-3.jpg](https://immunologicalsciences.com/wp-content/uploads/2023/02/AB-82949-3.jpg)
Immunohistochemistry of paraffinembedded human esophageal cancer using VEGFB Rabbit Ab at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.