VEGFR1 Rabbit Polyclonal Antibody
| Cat Number: | AB-84736 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1180 of human VEGFR1. |
| Reactivity: | Human, Rat, Mouse |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 200kDa |
| Purification: | Affinity purification |
| Synonyms: | FLT;FLT-1;VEGFR-1;VEGFR1;FLT1 |
| Background: | This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of extracts of various cell lines, using VEGFR1 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using VEGFR1 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using VEGFR1 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.