ZAP-70 Rabbit Polyclonal Antibody

Cat Number: AB-84734
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human ZAP-70. AA range:286-335
Reactivity: Human, Mouse, Rat

Western Blot: 1/500 – 1/2000
Immunohistochemistry: 1/100 – 1/300
Immunofluorescence: 1/200 – 1/1000
ELISA: 1/10000

Molecular Weight: 70kDa
Purification: Affinity purification
Synonyms: ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Zeta chain of T cell receptor associated protein kinase 70(ZAP70) Homo sapiens This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.

Western blot analysis of lysates from Jurkat cells, using ZAP-70 Antibody. The lane on the right is blocked with the synthesized peptide.


Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZAP-70 Antibody. The picture on the right is blocked with the synthesized peptide.


Immunofluorescence analysis of Jurkat cells using ZAP70 Rabbit pAb at
dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.