53BP1

Western blot analysis of extracts of A-431 cells, using 53BP1 antibody
 at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST. Detection: ECL West Pico Plus. 
Exposure time: 40s.

Western blot analysis of extracts of A-431 cells, using 53BP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 40s.

Catalog Number:AB-84202
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1
Reactivity:Hu, Ms, Rt
Applications:WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200
Molecular Weight:255kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:TP53BP1;53BP1;TDRD30;TP53;p202;P53bp1

Applications

WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1

Target Background

Synonyms:TP53BP1;53BP1;TDRD30;TP53;p202;P53bp1

Storage

Store at -20℃. Avoid freeze -thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of A-431 cells, using 53BP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 40s.

Immunohistochemistry of paraffin embedded rat kidney using 53BP1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse heart using 53BP1 antibody at dilution of 1:100 (40x lens).

No documents available.